Toyota Highlander Service Manual: Sliding roof/convertible

Engine immobilizer system

REGISTRATION 1. DESCRIPTION OF CODE REGISTRATION (a) When adding master keys and sub-keys (additional registration).  Register the key code (immobilizer code) in the transponder key ECU. ...

Sliding roof system

...

More about Toyota Highlander:

Using the mechanical key (vehicles with a smart key system)
To take out the mechanical key, push the release button and take the key out. The mechanical key can only be inserted in one direction, as the key only has grooves on one side. If the key cannot be inserted in a lock cylinder, turn it over and reattempt to insert it. After using the m ...